SrasV1, Main, Exploration, bibRecord, 003107

Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.

Identifieur interne : 003107 ( Main/Exploration ); précédent : 003106; suivant : 003108

Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.

Auteurs : Klara Kristin Eriksson [Suisse] ; Divine Makia ; Volker Thiel

Source :

Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2008.

RBID : pubmed:19057873

Descripteurs français

KwdFr :
- ARN viral (génétique), Animaux, Coronavirus (génétique), Coronavirus humain 229E (génétique), Humains, Lignée cellulaire, Modèles génétiques, Recombinaison génétique, Réplicon (génétique), Vecteurs génétiques (génétique), Virus de la vaccine (génétique).
MESH :
- génétique : ARN viral, Coronavirus, Coronavirus humain 229E, Réplicon, Vecteurs génétiques, Virus de la vaccine.
- Animaux, Humains, Lignée cellulaire, Modèles génétiques, Recombinaison génétique.

English descriptors

KwdEn :
- Animals, Cell Line, Coronavirus (genetics), Coronavirus 229E, Human (genetics), Genetic Vectors (genetics), Humans, Models, Genetic, RNA, Viral (genetics), Recombination, Genetic, Replicon (genetics), Vaccinia virus (genetics).
MESH :
- chemical , genetics : RNA, Viral.
- genetics : Coronavirus, Coronavirus 229E, Human, Genetic Vectors, Replicon, Vaccinia virus.
- Animals, Cell Line, Humans, Models, Genetic, Recombination, Genetic.

Abstract

Coronavirus reverse genetic systems have become valuable tools for studying the molecular biology of coronavirus infections. They have been applied to the generation of recombinant coronaviruses, selectable replicon RNAs, and coronavirus-based vectors for heterologous gene expression. Here we provide a collection of protocols for the generation, cloning, and modification of full-length coronavirus cDNA using vaccinia virus as a cloning vector. Based on cloned coronaviral cDNA, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229E. However, it is also applicable to the generation of other coronaviruses, such as the avian infectious bronchitis virus, mouse hepatitis virus, and SARS coronavirus.

DOI: 10.1007/978-1-59745-181-9_18
PubMed: 19057873

Affiliations:

Suisse

Le document en format XML

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<front><div type="abstract" xml:lang="en">Coronavirus reverse genetic systems have become valuable tools for studying the molecular biology of coronavirus infections. They have been applied to the generation of recombinant coronaviruses, selectable replicon RNAs, and coronavirus-based vectors for heterologous gene expression. Here we provide a collection of protocols for the generation, cloning, and modification of full-length coronavirus cDNA using vaccinia virus as a cloning vector. Based on cloned coronaviral cDNA, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229E. However, it is also applicable to the generation of other coronaviruses, such as the avian infectious bronchitis virus, mouse hepatitis virus, and SARS coronavirus.</div>
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Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.

Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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